Juq-139 Online

Recombinant human PI3K‑α (p110α/p85α) and isoforms β, δ, γ were purchased from SignalChem. Enzyme activity was measured using a homogeneous time‑resolved fluorescence (HTRF) assay (Kinase‑Glo, Promega). IC₅₀ values were derived from four‑parameter logistic fits (GraphPad Prism 9).

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